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Identifying kinetically stable proteins with capillary electrophoresis
Author(s) -
Zhang Songjie,
Xia Ke,
Chung Wai Keen,
Cramer Steven M.,
Colón Wilfredo
Publication year - 2010
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.336
Subject(s) - sodium dodecyl sulfate , chemistry , capillary electrophoresis , gel electrophoresis , polyacrylamide gel electrophoresis , biochemistry , electrophoresis , biophysics , chromatography , enzyme , biology
Abstract Unlike most proteins, which are in equilibrium with partially and globally unfolded conformations, kinetically stable proteins (KSPs) are trapped in their native conformations and are often resistant to harsh environment. Based on a previous correlation between kinetic stability (KS) and a protein's resistance to sodium dodecyl sulfate (SDS), we show here a simple method to identify KSPs by SDS‐capillary electrophoresis (CE). Control non‐KSPs were fully denatured by SDS and formed protein:SDS complexes that exhibited similar mobility in CE. In contrast, KSPs bound fewer SDS molecules, and showed a very different migration time and peak pattern in CE, thereby providing some insight about the structural heterogeneity of SDS:protein complexes and the relative KS of the various proteins.