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Cyclic voltammetry: A new strategy for the evaluation of oxidative damage to bovine insulin
Author(s) -
Zong Wansong,
Liu Rutao,
Sun Feng,
Wang Meijie,
Zhang Pengjun,
Liu Yihong,
Tian Yanmin
Publication year - 2010
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.313
Subject(s) - oxidative damage , oxidative phosphorylation , chemistry , cyclic voltammetry , insulin , sulfenic acid , sulfonic acid , cysteine , biochemistry , oxidative stress , electrochemistry , electrode , organic chemistry , medicine , enzyme
Abstract Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond SS CYS7A,CYS7B , forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO 2 H and sulfonic acid RSO 3 H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin.