Measurement of protein size in concentrated solutions by small angle X‐ray scattering | Zendy

Liu Jun | Zendy; Li Zhihong | Zendy; Wei Yanru | Zendy; Wang Wenjia | Zendy; Wang Bing | Zendy; Liang Hongli | Zendy; Gao Yuxi | Zendy

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Measurement of protein size in concentrated solutions by small angle X‐ray scattering

Author(s) -

Liu Jun,

Li Zhihong,

Wei Yanru,

Wang Wenjia,

Wang Bing,

Liang Hongli,

Gao Yuxi

Publication year - 2016

Publication title -

protein science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.353

H-Index - 175

eISSN - 1469-896X

pISSN - 0961-8368

DOI - 10.1002/pro.2957

Subject(s) - small angle x ray scattering , scattering , dispersity , small angle scattering , particle size , myoglobin , physics , materials science , molecular physics , crystallography , optics , chemistry , polymer chemistry , organic chemistry

By simulations on the distance distribution function (DDF) derived from small angle X‐ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.

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