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Measurement of protein size in concentrated solutions by small angle X‐ray scattering
Author(s) -
Liu Jun,
Li Zhihong,
Wei Yanru,
Wang Wenjia,
Wang Bing,
Liang Hongli,
Gao Yuxi
Publication year - 2016
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2957
Subject(s) - small angle x ray scattering , scattering , dispersity , small angle scattering , particle size , myoglobin , physics , materials science , molecular physics , crystallography , optics , chemistry , polymer chemistry , organic chemistry
By simulations on the distance distribution function (DDF) derived from small angle X‐ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.