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TRIC: Capturing the direct cellular targets of promoter‐bound transcriptional activators
Author(s) -
Dugan Amanda,
Pricer Rachel,
Katz Micah,
Mapp Anna K.
Publication year - 2016
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2951
Subject(s) - coactivator , chromatin immunoprecipitation , multiprotein complex , chemistry , activator (genetics) , promoter , transcription (linguistics) , transcription factor , microbiology and biotechnology , yeast , chromatin , biochemistry , gene expression , gene , biology , linguistics , philosophy
Transcriptional activators coordinate the dynamic assembly of multiprotein coactivator complexes required for gene expression to occur. Here we combine the power of in vivo covalent chemical capture with p‐benzoyl‐L‐phenylalanine (Bpa), a genetically incorporated photo‐crosslinking amino acid, and chromatin immunoprecipitation (ChIP) to capture the direct protein interactions of the transcriptional activator VP16 with the general transcription factor TBP at the GAL1 promoter in live yeast.