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A critical element of the light‐induced quaternary structural changes in Y tv A ‐ LOV
Author(s) -
Lee Rang,
Gam Jongsik,
Moon Jayoung,
Lee SeungGoo,
Suh YoungGer,
Lee BongJin,
Lee Jeeyeon
Publication year - 2015
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2810
Subject(s) - dimer , chemistry , biophysics , monomer , helix (gastropod) , flavin mononucleotide , flavin group , stereochemistry , crystallography , biochemistry , biology , enzyme , ecology , organic chemistry , snail , polymer
Abstract YtvA, a photosensory LOV (light‐oxygen‐voltage) protein from Bacillus subtilis , exists as a dimer that previously appeared to undergo surprisingly small structural changes after light illumination compared with other light‐sensing proteins. However, we now report that light induces significant structural perturbations in a series of YtvA‐LOV domain derivatives in which the Jα helix has been truncated or replaced. Results from native gel analysis showed significant mobility changes in these derivatives after light illumination; YtvA‐LOV without the Jα helix dimerized in the dark state but existed as a monomer in the light state. The absence of the Jα helix also affected the dark regeneration kinetics and the stability of the flavin mononucleotide (FMN) binding to its binding site. Our results demonstrate an alternative way of photo‐induced signal propagation that leads to a bigger functional response through dimer/monomer conversions of the YtvA‐LOV than the local disruption of Jα helix in the As‐LOV domain.