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Mispairs with Watson‐Crick base‐pair geometry observed in ternary complexes of an RB69 DNA polymerase variant
Author(s) -
Xia Shuangluo,
Konigsberg William H.
Publication year - 2014
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2434
Subject(s) - molecular structure of nucleic acids: a structure for deoxyribose nucleic acid , base pair , ternary operation , dna polymerase , dna , base (topology) , physics , chemistry , geometry , genetics , biology , mathematics , computer science , mathematical analysis , programming language
Recent structures of DNA polymerase complexes with dGMPCPP/dT and dCTP/dA mispairs at the insertion site have shown that they adopt Watson‐Crick geometry in the presence of Mn 2+ indicating that the tautomeric or ionization state of the base has changed. To see whether the tautomeric or ionization state of base‐pair could be affected by its microenvironment, we determined 10 structures of an RB69 DNA polymerase quadruple mutant with dG/dT or dT/dG mispairs at position n‐1 to n‐5 of the Primer/Template duplex. Different shapes of the mispairs, including Watson‐Crick geometry, have been observed, strongly suggesting that the local environment of base‐pairs plays an important role in their tautomeric or ionization states.