Premium
The prohormone proenkephalin possesses differential conformational features of subdomains revealed by rapid H‐D exchange mass spectrometry
Author(s) -
Lu Weiya D.,
Liu Tong,
Li Sheng,
Woods Virgil L.,
Hook Vivian
Publication year - 2012
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.2000
Subject(s) - chemistry , hydrogen–deuterium exchange , prohormone , proenkephalin , cleavage (geology) , peptide , mass spectrometry , biochemistry , enkephalin , chromatography , hormone , biology , paleontology , receptor , fracture (geology) , opioid
Active enkephalin and related peptide hormones or neurotransmitters are generated by proteolytic processing of inactive prohormone precursors. Little is known about the relative accessibilities of prohormone cleavage sites and conformations of subdomains that undergo proteolytic processing. Therefore, this study investigated the conformational features of the prohormone proenkephalin (PE) by rapid hydrogen‐deuterium exchange mass spectrometry (DXMS). DXMS analyzes rates of hydrogen exchange of the polypeptide backbone of PE with deuterium from D 2 O (heavy water) by mass spectrometry, accomplished at sub‐second and multisecond time periods. Results showed differential accessibilities of cleavage sites and adjacent subdomains of PE to the aqueous environment. Importantly, protease cleavage sites of PE with greater relative accessibilities correspond to sites most readily cleaved by processing proteases to generate active peptide neurotransmitters. For comparison, peptides derived from PE (by pepsin digestion) displayed greater accessibility to the solvent environment, illustrated by their higher rates of H‐D exchange compared to that of intact PE protein. The more limited H‐D exchange accessibilities of PE protein, compared to peptides derived from PE, indicate that PE possesses tertiary conformation. These results demonstrate that differential tertiary conformations of PE subdomains undergo ordered proteolytic processing to generate active enkephalin peptides for cell‐cell communication in the nervous and endocrine systems.