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The thermo‐ and acido‐stable ORF‐99 from the archaeal virus AFV1
Author(s) -
Goulet Adeline,
Spinelli Silvia,
Blangy Stéphanie,
van Tilbeurgh Herman,
Leulliot Nicolas,
Basta Tamara,
Prangishvili David,
Cambillau Christian,
Campanacci Valérie
Publication year - 2009
Publication title -
protein science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.353
H-Index - 175
eISSN - 1469-896X
pISSN - 0961-8368
DOI - 10.1002/pro.122
Subject(s) - archaea , virus , chemistry , amino acid , dna , biochemistry , dna virus , genome , biology , virology , gene
Acidianus Filamentous Virus 1 (AFV1), isolated from acidic hot springs, is an enveloped lipid‐containing archaeal filamentous virus with a linear double‐stranded DNA genome. It infects Acidianus , which is a hyperthermostable archaea growing at 85°C and acidic pHs, below pH 3. AFV1‐99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families Lipothrixviridae and Rudiviridae. We determined the crystal structure of AFV1‐99 at 2.05 Å resolution. AFV1‐99 has a new fold, is hyperthermostable (up to 95°C) and resists to extreme pH (between pH 0 and 11) and to the combination of high temperature (95°C) and low pH (pH 0). It possesses characteristics of hyperthermostable proteins, such as a high content of charged residues.