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Mass spectrometry‐based proteomic analysis of the effect of storage temperature on non‐invasively collected samples of human stratum corneum
Author(s) -
Azimi Ali,
Teh Rachel,
FernandezPenas Pablo
Publication year - 2021
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.202100005
Subject(s) - stratum corneum , proteome , mass spectrometry , chromatography , chemistry , biomarker discovery , proteomics , sample preparation , analytical chemistry (journal) , biochemistry , medicine , pathology , gene
Purpose The collection of human stratum corneum (SC) samples by tape‐stripping promises to deliver a non‐invasive method for biomarker analysis of skin diseases. The current study examines the effect of storage temperature and SC layer depth on the proteome profile of SC samples. Experimental Design The samples were collected from healthy volunteers ( n = 5) using 10 sequential adhesive discs. Discs were pooled by five (discs 1–5, 6–10) and stored at various temperatures for 10 days before their analysis by mass spectrometry. Results No statistically significant difference was seen in the protein yield between discs 1–5 (14.8 mg) and 6–10 (14.4 mg), or between discs stored at ‐80°C (14.7 mg), ‐20°C (15.8 mg), 4°C (14.9 mg) or room temperature (13.2 mg). Mass spectrometry analysis revealed that the storage of SC samples at higher temperatures does not affect their proteome profile considerably (< 4.7% peptide precursor loss at lower temperatures vs. ‐80°C). On the other hand, while 95.3% of the identified peptide precursors were shared between discs 1–5 and 6–10, the level of 17 proteins was significantly changed between these conditions. Conclusions The findings of this study will likely have major implications on the conduct of proteomic studies involving SC sample collection, storage, and transportation.