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Salivary Fingerprinting of Periodontal Disease by Infrared‐ATR Spectroscopy
Author(s) -
BeyerHans Kerstin MetaCatherina,
Sigrist Markus Werner,
Silbereisen Angelika,
Ozturk Veli Ozgen,
Emingil Gulnur,
Bostanci Nagihan
Publication year - 2020
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201900092
Subject(s) - saliva , periodontitis , periodontology , chronic periodontitis , attenuated total reflection , medicine , aggressive periodontitis , periodontal disease , dentistry , chemistry , infrared spectroscopy , organic chemistry
Purpose Periodontal diseases, the most common chronic inflammatory diseases in humans, do not only affect tooth‐supporting tissues but also other body parts by contributing to the development of life‐threatening conditions. Since currently available diagnostic methods in periodontics lack the ability to identify patients at high risk for periodontal disease progression, development of innovative, non‐invasive, rapid detection methods for diagnosing periodontal diseases is needed. This study aims to assess the potential of infrared attenuated total reflection (IR‐ATR) spectroscopy to detect differences in composition of saliva supernatant in non‐periodontitis individuals (control) and patients with generalized aggressive periodontitis (G‐AgP). Experimental Design IR‐ATR is performed with a wavelength interval from 1230 to 1180 cm −1 , analyzed with a simple subtraction in absorbance data. Results Ten samples show in the analysis of variance of the two data sets a true difference (99.8%). A principal component analysis (PCA) is able to discriminate between G‐AgP and control groups. Conclusion and Clinical Relevance This study demonstrates for the first time that IR‐ATR spectroscopy is a promising tool for the analysis of saliva supernatant for the diagnosis of periodontitis, and potentially other periodontal conditions. IR‐ATR spectroscopy holds the potential to be miniaturized and utilized as a non‐invasive screening test.