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Transcriptomic and Proteomic Profiling of Human Mesenchymal Stem Cell Derived from Umbilical Cord in the Study of Preterm Birth
Author(s) -
Chien ChihWei,
Lo YuShu,
Wu HsinYi,
Hsuan Yogi,
Lin ChiKang,
Chen YuJu,
Lin Willie,
Han ChiaLi
Publication year - 2020
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201900024
Subject(s) - transcriptome , mesenchymal stem cell , umbilical cord , proteomics , omics , biology , microarray , bioinformatics , proteome , gene expression profiling , gene expression , gene , immunology , microbiology and biotechnology , genetics
Objective Mesenchymal stem cells (MSCs) hold great therapeutic potential in morbidities associated with preterm birth. However, the molecular expressions of MSCs in preterm birth infants are not systematically evaluated. In this study, the dual‐omics analyses of umbilical‐cord (UC)‐derived MSCs to identify the dysregulated cellular functions are presented. Materials and methods The UC‐MSCs are collected from ten full‐term and eight preterm birth infants for microarray and iTRAQ‐based proteome profiling. Results The integrative analysis of dual‐omics data discovered 5615 commonly identified genes/proteins of which 29 genes/proteins show consistent up‐ or downregulation in preterm birth. The Gene Ontology analysis reveals that dysregulation of mitochondrial translation and cellular response to oxidative stress are mainly enriched in 290 differential expression proteins (DEPs) while the 412 differential expression genes (DEGs) are majorly involved in single‐organism biosynthetic process, cellular response to stress, and mitotic cell cycle in preterm birth. Besides, a 13‐protein module involving CUL2 and CUL3 is identified, which plays an important role in cullin‐RING‐based ubiquitin ligase complex, as potential mechanism for preterm birth. Conclusion The dual‐omics data not only provide new insights to the molecular mechanism but also identify panel of candidate markers associated with preterm birth.