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Back Cover: MALDI Imaging‐Guided Microproteomic Analyses of Heterogeneous Breast Tumors—A Pilot Study
Author(s) -
Alberts Deborah,
Pottier Charles,
Smargiasso Nicolas,
Baiwir Dominique,
Mazzucchelli Gabriel,
Delvenne Philippe,
Kriegsmann Mark,
Kazdal Daniel,
Warth Arne,
Pauw Edwin,
Longuespée Rémi
Publication year - 2018
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201870005
Subject(s) - maldi imaging , laser capture microdissection , breast cancer , pathology , biology , matrix assisted laser desorption/ionization , medicine , cancer , chemistry , biochemistry , gene expression , organic chemistry , adsorption , desorption , gene
Matrix‐assisted laser desorption ionisation (MALDI)‐imaging coupled to laser microdissection (LMD)‐based microproteomics to study intratumor heterogeneity. MALDI imaging dataset from breast cancer FFPE tissue sections were segmented to draw intratumor proteomic heterogeneity. Tissue regions containing 3500 cells from different molecular clusters were collected by LMD and processed for further microproteomic analyses. The combination of MALDI imaging with LMD‐based microproteomics will pave the way to in‐depth characterization of intratumor heterogeneity in pathology. This is reported by Deborah Alberts, Charles Pottier, Nicolas Smargiasso, Dominique Baiwir, Gabriel Mazzucchelli, Philippe Delvenne, Mark Kriegsmann, Daniel Kazdal, Arne Warth, Edwin De Pauw and Rémi Longuespée, article number 1700062.