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Changes in Protein Glycosylation as a Result of Aptamer Interactions with Cancer Cells
Author(s) -
Drabik Anna,
NerKluza Joanna,
Hartman Kinga,
Mayer Günter,
Silberring Jerzy
Publication year - 2020
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201800186
Subject(s) - aptamer , glycan , computational biology , epitope , cancer , oligonucleotide , glycosylation , glycobiology , biology , systematic evolution of ligands by exponential enrichment , cancer cell , cancer research , chemistry , antibody , microbiology and biotechnology , biochemistry , glycoprotein , immunology , gene , rna , genetics
Purpose Based on the recent aptamer‐related breast cancer studies, which indicate the therapeutic role of specific oligonucleotide sequences, experiments have been designed in an attempt to unravel the molecular targets of this mechanism. This article describes the study on glycoproteome changes in breast cancer cells as a result of their interactions with aptamers. Experimental design Aberrations in protein glycosylation play an important role in tumorigenesis and influence cancer progression, metastasis, immunoresponse, and chemoresistance, therefore this study is focused on the identification of the alterations in glycan expression on the surface of proteins as a potential and innovative tool for biomedical applications of aptamers in cancer treatment. Results Two proteins, kinesin‐like protein (KI13B) and proliferating cell nuclear antigen (PCNA), have been identified that carry N ‐glycan epitopes after conjugation with aptamer sequences. Conclusions and clinical relevance Multiple features of aptamers as an alternative to protein antibodies are utilized for various biomedical applications ranging from biomarker discovery, bioimaging, targeted therapy, drug delivery, and drug pharmacokinetics and biodistribution. Frequently, aptamers bind to their target molecules and modulate their function. Such therapeutic aptamers can modify the biological pathways for treatment of many types of diseases, such as cancer.