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Stearate‐Induced Apoptosis in Human Pancreatic β‐Cells is Associated with Changes in Membrane Protein Expression and These Changes are Inhibited by Oleate
Author(s) -
NěmcováFürstová Vlasta,
Balušíková Kamila,
Halada Petr,
Pavlíková Nela,
Šrámek Jan,
Kovář Jan
Publication year - 2019
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201800104
Subject(s) - annexin , stearate , apoptosis , lipotoxicity , programmed cell death , viability assay , western blot , biochemistry , biology , microbiology and biotechnology , chemistry , endocrinology , diabetes mellitus , gene , insulin resistance , organic chemistry
Purpose Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic β‐cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. Experimental design Employing 2DE analysis and subsequent western blot confirmation, the differences in membrane/membrane‐associated protein expression in human β‐cell line NES2Y are assessed during cell death induction by stearate and its inhibition by oleate. Results Induction of apoptosis by stearate is associated with significantly increased levels of Hsp90β, peroxiredoxin‐1, and 14‐3‐3γ in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin‐2. All these changes are significantly inhibited by oleate co‐application. No expression changes are detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin‐2 is significantly decreased after stearate application also in the whole cell lysate. Conclusions and clinical relevance Several membrane‐associated proteins that could be related to pro‐ and anti‐apoptotic signaling initiated by fatty acids in human pancreatic β‐cells are identified. As far as we know, annexin A4, reticulocalbin‐2, and 14‐3‐3γ represent novel molecules related to the effect of fatty acids on β‐cell viability.