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Matrix‐Assisted Laser Desorption Ionization Mass Spectrometry Imaging of Key Proteins in Corneal Samples from Lattice Dystrophy Patients with TGFBI ‐H626R and TGFBI ‐R124C Mutations
Author(s) -
Venkatraman Anandalakshmi,
Hochart Guillaume,
Bonnel David,
Stauber Jonathan,
Shimmura Shigeto,
Rajamani Lakshminaryanan,
Pervushin Konstantin,
Mehta Jodhbir S.
Publication year - 2019
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201800053
Subject(s) - tgfbi , cornea , corneal dystrophy , laser capture microdissection , chemistry , mass spectrometry imaging , pathology , microbiology and biotechnology , biology , extracellular matrix , mass spectrometry , medicine , biochemistry , gene , ophthalmology , gene expression , chromatography
Scope The purpose of this study is to identify and visualize the spatial distribution of proteins present in amyloid corneal deposits of TGFBI‐CD patients using Mass Spectrometry Imaging (MSI) and compare it with healthy control cornea. Corneal Dystrophies (CD) constitute a group of genetically inherited protein aggregation disorders that affects different layers of the cornea. With accumulated protein deposition, the cornea becomes opaque with decreased visual acuity. CD affecting the stroma and Bowman's membrane, is associated with mutations in transforming growth factor β‐induced ( TGFBI ) gene. Methods MALDI‐Mass Spectrometry Imaging (MSI) is performed on 2 patient corneas and is compared with 1 healthy control cornea using a 7T‐MALDI‐FTICR. Molecular images obtained are overlaid with congo‐red stained sections to visualize the proteins associated with the corneal amyloid aggregates. Results MALDI‐MSI provides a relative abundance and two dimensional spatial protein signature of key proteins (TGFBIp, Apolipoprotein A‐I, Apolipoprotein A‐IV, Apolipoprotein E, Kaliocin‐1, Pyruvate Kinase and Ras related protein Rab‐10) in the patient deposits compared to the control. This is the first report of the anatomical localization of key proteins on corneal tissue section from CD patients. This may provide insight in understanding the mechanism of amyloid fibril formation in TGFBI ‐corneal dystrophy.