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Development of a Class Prediction Model to Discriminate Pancreatic Ductal Adenocarcinoma from Pancreatic Neuroendocrine Tumor by MALDI Mass Spectrometry Imaging
Author(s) -
Casadonte Rita,
Kriegsmann Mark,
Perren Aurel,
Baretton Gustavo,
Deininger SörenOliver,
Kriegsmann Katharina,
Welsch Thilo,
Pilarsky Christian,
Kriegsmann Jörg
Publication year - 2019
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201800046
Subject(s) - medicine , pancreatic cancer , pancreatic ductal adenocarcinoma , maldi imaging , pathology , differential diagnosis , adenocarcinoma , matrix assisted laser desorption/ionization , oncology , cancer , chemistry , organic chemistry , adsorption , desorption
Purpose To define proteomic differences between pancreatic ductal adenocarcinoma (pDAC) and pancreatic neuroendocrine tumor (pNET) by matrix‐assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Experimental design Ninety‐three pDAC and 126 pNET individual tissues are assembled in tissue microarrays and analyzed by MALDI MSI. The cohort is separated in a training (52 pDAC and 83 pNET) and validation set (41 pDAC and 43 pNET). Subsequently, a linear discriminant analysis (LDA) model based on 46 peptide ions is performed on the training set and evaluated on the validation cohort. Additionally, two liver metastases and a whole slide of pDAC are analyzed by the same LDA algorithm. Results Classification of pDAC and pNET by the LDA model is correct in 95% (39/41) and 100% (43/43) of patients in the validation cohort, respectively. The two liver metastases and the whole slide of pDAC are also correctly classified in agreement with the histopathological diagnosis. Conclusion and clinical relevance In the present study, a large dataset of pDAC and pNET by MALDI MSI is investigated, a class prediction model that allowed separation of both entities with high accuracy is developed, and differential peptide peaks with potential diagnostic, prognostic, and predictive values are highlighted.