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Integrated Multi‐Omic Analyses Support Distinguishing Secretory Carcinoma of the Breast from Basal‐Like Triple‐Negative Breast Cancer
Author(s) -
Jin MinSun,
Lee Hyebin,
Woo Jongmin,
Choi Seongmin,
Do Mi Sol,
Kim Kwangsoo,
Song Min Ji,
Kim Youngsoo,
Park In Ae,
Han Dohyun,
Ryu Han Suk
Publication year - 2018
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700125
Subject(s) - triple negative breast cancer , breast cancer , proteomics , exome sequencing , biology , breast carcinoma , cancer research , basal (medicine) , cancer , computational biology , clinical significance , oncology , bioinformatics , medicine , mutation , gene , genetics , endocrinology , insulin
Purpose Secretory carcinoma (SC) of the breast is defined as an indolent tumor but is still categorized into a basal‐like triple‐negative breast cancer (BL‐TNBC) subgroup that generally shows aggressive behavior according to the current classification. Despite the unique clinical behavior of SC, molecular characteristics that reflect biological behaviors of SC remain largely unknown. Experimental design A combinatorial approach of whole‐exome sequencing and mass spectrometry–based in‐depth quantitative proteomics to determine the entire molecular landscape of SC using three SC formalin‐fixed paraffin‐embedded (FFPE) tissues is employed. Results Exome sequencing and proteomic analysis of SC identified 419 unique somatic mutations and 721 differentially expressed proteins as compared with triple‐negative breast cancer (TNBC), respectively. Several pathways related to cancer metabolism were significantly upregulated in the SC group. Comparative analyses with multiple datasets revealed that SC shares genomic mutations and biological pathways more closely related to hormone receptor–positive breast cancer than BL‐TNBC. Conclusion and clinical relevance These multi‐omic analyses provide evidence that SC harbors substantially different molecular genomic and proteomic landscapes compared with BL‐TNBC. These results provide an entire spectrum of in‐depth molecular landscapes to support the hypothesis that SC is distinct from BL‐TNBC.