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A 14‐Protein Signature for Rapid Identification of Poor Prognosis Stage III Metastatic Melanoma
Author(s) -
Sykes Erin K.,
McDonald Cassandra E.,
Ghazanfar Shila,
Mactier Swetlana,
Thompson John F.,
Scolyer Richard A.,
Yang Jean Y.,
Mann Graham J.,
Christopherson Richard I.
Publication year - 2018
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700094
Subject(s) - melanoma , medicine , stage (stratigraphy) , oncology , adjuvant therapy , cancer , survival analysis , biomarker , proportional hazards model , cancer research , biology , paleontology , biochemistry
Purpose To validate differences in protein levels between good and poor prognosis American Joint Committee on Cancer (AJCC) stage III melanoma patients and compile a protein panel to stratify patient risk. Experimental design Protein extracts from melanoma metastases within lymph nodes in patients with stage III disease with good ( n = 16, >4 years survival) and poor survival ( n = 14, <2 years survival) were analyzed by selected reaction monitoring (SRM). Diagonal Linear Discriminant Analysis (DLDA) was performed to generate a protein biomarker panel. Results SRM analysis identified ten proteins that were differentially abundant between good and poor prognosis stage III melanoma patients. The ten differential proteins were combined with 22 proteins identified in our previous work. A panel of 14 proteins was selected by DLDA that was able to accurately classify patients into prognostic groups based on levels of these proteins. Conclusions and clinical relevance The ten differential proteins identified by SRM have biological significance in cancer progression. The final signature of 14 proteins identified by SRM could be used to identify AJCC stage III melanoma patients likely to have poor outcomes who may benefit from adjuvant systemic therapy.