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Evolution of the plasma proteome of divers before and after a single SCUBA dive
Author(s) -
Lautridou Jacky,
Pichereau Vianney,
Artigaud Sébastien,
Bernay Benoit,
Barak Otto,
Hoiland Ryan,
Lovering Andrew T.,
Eftedal Ingrid,
Dujic Zeljko,
Guerrero François
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700016
Subject(s) - proteome , transthyretin , decompression , decompression sickness , blood proteins , abundance (ecology) , two dimensional gel electrophoresis , chemistry , medicine , andrology , biology , proteomics , surgery , biochemistry , ecology , gene
Purpose Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms. Experimental design Twelve healthy male divers were subjected to a single dive at a depth of 18 m of sea water (msw) with a 47‐min bottom time followed by a direct ascent to the surface at a rate of 9 msw/min. Venous blood was collected before the dive as well as 30 min and 2 h following the dive. The plasma proteomes from four individuals were then analyzed by using a two‐dimensional electrophoresis‐based proteomic strategy. Results No protein spot showed a significantly changed abundance (fdr< 0.1) between the tested times. Conclusion These results strengthen the hypothesis according to which significant changes of the plasma proteome measurable with two‐dimensional electrophoresis may only occur along with DCS symptoms.