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Quantitative proteome analysis of plasma microparticles for the characterization of HCV‐induced hepatic cirrhosis and hepatocellular carcinoma
Author(s) -
Taleb Raghda Saad Zaghloul,
Moez Pacint,
Younan Doreen,
Eisenacher Martin,
Tenbusch Matthias,
Sitek Barbara,
Bracht Thilo
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700014
Subject(s) - hepatocellular carcinoma , cirrhosis , proteome , hepatitis c virus , medicine , liver cancer , liver disease , hepatitis c , cancer , proteomics , carcinogenesis , cancer research , immunology , virus , biology , bioinformatics , gene , biochemistry
Purpose Hepatocellular carcinoma (HCC) is the most common primary malignant liver tumor and a leading cause of cancer‐related deaths worldwide. Cirrhosis induced by hepatitis‐C virus (HCV) infection is the most critical risk factor for HCC. However, the mechanism of HCV‐induced carcinogenesis is not fully understood. Plasma microparticles (PMP) contribute to numerous physiological and pathological processes and contain proteins whose composition correlates to the respective pathophysiological conditions. Experimental design We analyzed PMP from 22 HCV‐induced cirrhosis patients, 16 HCV‐positive HCC patients with underlying cirrhosis and 18 healthy controls. PMP were isolated using ultracentrifugation and analyzed via label‐free LC‐MS/MS. Results We identified 840 protein groups and quantified 507 proteins. 159 proteins were found differentially abundant between the three experimental groups. PMP in both disease entities displayed remarkable differences in the proteome composition compared to healthy controls. Conversely, the proteome difference between both diseases was minimal. GO analysis revealed that PMP isolated from both diseases were significantly enriched in proteins involved in complement activation, while endopeptidase activity was downregulated exclusively in HCC patients. Conclusion This study reports for the first time a quantitative proteome analysis for PMP from patients with HCV‐induced cirrhosis and HCC. Data are available via ProteomeXchange with identifier PXD005777.