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Screening of antigenic vesicular fluid proteins of Echinococcus multilocularis as potential viability biomarkers to monitor drug response in alveolar echinococcosis patients
Author(s) -
Valot Benoît,
Rog Bénédicte,
Prenel Anais,
Baraquin Alice,
Knapp Jenny,
Anelli Mathilde,
Richou Carine,
BressonHadni Solange,
Grenouillet Frederic,
Wang Junhua,
Vuitton Dominique Angèle,
Gottstein Bruno,
Millon Laurence
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700010
Subject(s) - echinococcus multilocularis , alveolar echinococcosis , echinococcosis , antigen , echinococcus , immunology , biology , virology , microbiology and biotechnology , medicine , pathology
Purpose The only drugs available to treat alveolar echinococcosis (AE) are mostly parasitostatic and in many cases prescribed for life. Decision criteria for discontinuation rely on the absence of parasitic viability. The aim of the present study is to search for candidate proteins that may exhibit good potential as biomarkers for viability. Experimental design Sixteen serum samples (five healthy controls, 11 patients with AE), are used. AE‐patients are classified into three groups “Cured” ( n = 2), “ABZ‐responders” ( n = 4) and “ABZ‐nonresponders” ( n = 5). Immunoreactive proteins from vesicular fluid (VF) are identified and quantified by LC‐MS/MS analysis after immunoprecipitation (IP) using all 16 serum samples. Results Shotgun analysis of VF lead to the identification of 107 E. multilocularis proteins. Comparative proteomics reveal nine proteins more abundant in IP eluates from ABZ‐nonresponder patients (cathepsin b, prosaposin a preprotein, actin modulator protein, fucosidase alpha L1 tissue, gluthatione‐S‐tranferase, beta galactosidase, elongation factor 2, H17g protein tegumental antigen, and NiemannPick C2 protein). Conclusions and clinical relevance Detection of antibodies against these proteins by ELISA could be helpful to monitor the course of alveolar echinococcosis under albendazole (ABZ) treatment.