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Altered signaling associated with chronic arsenic exposure in human skin keratinocytes
Author(s) -
Mir Sartaj Ahmad,
Renuse Santosh,
Sathe Gajanan,
Khan Aafaque Ahmad,
Patil Arun H.,
Nanjappa Vishalakshi,
Bhat Firdous Ahmad,
Prasad T.S. Keshava,
Giri Ashok K.,
Chatterjee Aditi,
Gowda Harsha
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201700004
Subject(s) - microbiology and biotechnology , hacat , stable isotope labeling by amino acids in cell culture , adherens junction , phosphoproteomics , ask1 , biology , map kinase kinase kinase , protein kinase a , kinase , signal transduction , phosphorylation , mitogen activated protein kinase kinase , protein phosphorylation , chemistry , proteomics , biochemistry , cell , cadherin , in vitro , gene
Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here, we carried out SILAC‐based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4171 unique phosphosites derived from 2000 proteins. We observed differential phosphorylation of 406 phosphosites (twofold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched ( p <0.05). Our data revealed altered phosphorylation of proteins associated with adherens junction remodeling and actin polymerization. Kinases such as protein kinase C iota type (PRKCI), mitogen‐activated protein kinase kinase kinase 1 (MAP3K1), tyrosine‐protein kinase BAZ1B (BAZ1B) and STE20 like kinase (SLK) were found to be hyperphosphorylated. Our study provides novel insights into signaling perturbations associated with chronic arsenic exposure in human skin keratinocytes. All MS/MS data have been deposited to the ProteomeXchange with identifier PXD004868.