Premium
Identification of salivary protein biomarkers for orthodontically induced inflammatory root resorption
Author(s) -
KaczorUrbanowicz Karolina Elżbieta,
Deutsch Omer,
Zaks Batia,
Krief Guy,
Chaushu Stella,
Palmon Aaron
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201600119
Subject(s) - blot , saliva , salivary proteins , root resorption , western blot , chemistry , dentistry , medicine , resorption , microbiology and biotechnology , pathology , biology , biochemistry , gene
Purpose Orthodontically induced inflammatory root resorption (OIIRR) is one of the most prevalent and unavoidable consequence of orthodontic tooth movement. The aim of this study was to discover potential diagnostic protein biomarkers for detection of OIIRR in whole saliva (WS). Material and methods Unstimulated WS was collected from 72 subjects: 48 OIIRR patients and 24 untreated, generally healthy, age and gender matched controls. Radiographic assessment of periapical x‐rays of four upper incisors taken before and 9 months after bonding was done. High‐abundance proteins were depleted followed by two‐dimensional‐gel‐electrophoresis and quantitative mass spectrometry (qMS). Finally, to initially validate qMS results, Western blotting was performed. Results qMS revealed differentially expressed proteins in the moderate‐to‐severe OIIRR group, which have never been found in WS before. Additionally, in the moderate‐to‐severe young OIIRR group, the pathogenetic mechanisms related to actin cytoskeleton regulation and Fc gamma R‐ mediated phagocytosis were detected, while in adults‐ to focal adhesion. Preliminary validation by Western blotting of fetuin‐A and p21‐ARC indicated expression profile trends similar to those identified by qMS. Conclusion The significance of WS novel proteomic methodologies is clearly demonstrated for detecting new OIIRR biomarkers as well as for unveiling possible novel pathogenetic mechanisms in both young and adult patients.