Premium
Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women
Author(s) -
Berg Caroline B.,
Duvekot Johannes J.,
Güzel Coşkun,
Hansson Stefan R.,
Leeuw Thomas G.,
Steegers Eric A. P.,
Versendaal Johannes,
Luider Theo M.,
Stoop Marcel P.
Publication year - 2017
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201600082
Subject(s) - cerebrospinal fluid , preeclampsia , medicine , proteome , pregnancy , hemoglobin , beta 2 microglobulin , endocrinology , andrology , bioinformatics , biology , genetics
Purpose To investigate the cerebrospinal fluid (CSF) proteome of patients with preeclampsia (PE) and normotensive pregnant women, in order to provide a better understanding of brain involvement in PE. Experimental design Ninety‐eight CSF samples (43 women with PE and 55 normotensive controls) were analyzed by LC–MS/MS proteome profiling. CSF was obtained during the spinal puncture before caesarean delivery. Results Eight proteins were higher abundant and 17 proteins were lower abundant in patients with PE. The most significantly differentially abundant protein was protein AMBP (alpha‐1‐microglobulin/bikunin precursor). This finding was validated by performing an ELISA experiment ( p = 0.002). Conclusions and clinical relevance The current study showed a clear difference between the protein profiles of CSF from patients with PE and normotensive pregnant women. Protein AMBP is a precursor of a heme‐binding protein that counteracts the damaging effects of free hemoglobin, which may be related to the presence of free hemoglobin in CSF. Protein levels showed correlations with clinical symptoms during pregnancy and postpartum. To our knowledge, this is the first LC–MS/MS proteome profiling study on a unique set of CSF samples from (severe) preeclamptic patients and normotensive pregnant women.