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Comparative proteomics of umbilical vein blood plasma from normal and gestational diabetes mellitus patients reveals differentially expressed proteins associated with childhood obesity
Author(s) -
Miao Zhijing,
Wang Jianqing,
Wang Fuqiang,
Liu Lan,
Ding Hongjuan,
Shi Zhonghua
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201600046
Subject(s) - ingenuity , gestational diabetes , offspring , umbilical vein , tandem mass tag , blood proteins , obesity , proteomics , blot , biology , endocrinology , pathway analysis , medicine , lipid metabolism , pregnancy , bioinformatics , quantitative proteomics , gestation , gene expression , biochemistry , genetics , gene , neoclassical economics , in vitro , economics
Purpose Offspring obesity is one of long‐term complications of gestational diabetes mellitus (GDM). The aim of this study is to identify proteins differentially expressed in the umbilical vein blood plasma, which could become markers for early diagnosis of childhood obesity. Experimental design Umbilical vein plasma samples were collected from 30 control and 30 GDM patients in 2007–2008 whose offspring were suffering from obesity at 6–7 years old. Multiplexed isobaric tandem mass tag labeling combined with LC–MS/MS was used to identify differentially expressed proteins. Ingenuity pathway analysis was performed to identify canonical pathways, biological functions, and networks of interacting proteins. Western blotting was used to verify the expression of three selected proteins. Results A total of 318 proteins were identified, of which 12 proteins were upregulated in GDM group while 24 downregulated. Lipid metabolism was the top category identified by ingenuity pathway analysis. Three randomly chosen proteins were validated by Western blotting, which were consistent with LC–MS. Conclusion There are significant differences of protein profile in the umbilical vein blood plasma between normal and GDM patients with obese offspring. The results indicate that a variety of proteins and biological mechanisms may contribute to childhood obesity.