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Proteomic profiling of Gram‐negative bacterial outer membrane vesicles: Current perspectives
Author(s) -
Lee Jaewook,
Kim Oh Youn,
Gho Yong Song
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201600032
Subject(s) - gram negative bacteria , bacterial outer membrane , profiling (computer programming) , gram , chemistry , biophysics , microbiology and biotechnology , bacteria , computational biology , biology , biochemistry , computer science , escherichia coli , genetics , gene , operating system
Outer membrane vesicles (OMVs) are extracellular vesicles derived from Gram‐negative bacteria. Recent progress in the studies of Gram‐negative bacterial extracellular vesicles implies that OMVs may function as intercellular communicasomes in bacteria–bacteria and bacteria–host interactions. Current MS‐based high‐throughput proteomic analyses of Gram‐negative bacterial OMVs have identified thousands of vesicular proteins and provided clues to reveal the biogenesis and pathophysiological functions of Gram‐negative bacterial OMVs. The future directions of proteomics of Gram‐negative bacterial OMVs may include the isolation strategy of Gram‐negative bacterial OMVs to thoroughly exclude nonvesicular contaminants and proteomics of Gram‐negative bacterial OMVs derived from diverse conditions as well as body fluids of bacterium‐infected hosts. We hope this review will shed light on future research in this emerging field of proteomics of extracellular vesicles derived from Gram‐negative bacteria and contribute to the development of OMV‐based diagnostic tools and effective vaccines.