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Protein translation occurs in platelet concentrates despite riboflavin/UV light pathogen inactivation treatment
Author(s) -
Schubert Peter,
Culibrk Brankica,
Karwal Simrath,
Goodrich Raymond P.,
Devine Dana V.
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201500139
Subject(s) - proteome , proteomics , puromycin , platelet , quantitative proteomics , riboflavin , pathogen , biology , translation (biology) , biochemistry , microbiology and biotechnology , protein biosynthesis , chemistry , messenger rna , immunology , gene
Purpose Pathogen inactivation technologies (PITs) were introduced into blood banking to further improve the safety of blood products. However, the UV light used in PITs to terminate pathogen growth might alter the functionality of the cells in the blood product as well as the protein profile of the blood components. This study employed proteomic approaches to assess changes in the platelet proteome and translatome. Experimental design Apheresis‐derived platelet concentrates treated with riboflavin/UV light or untreated controls were analyzed throughout blood bank storage by quantitative proteomics using iTRAQ and puromycin‐associated nascent chain (PUNCH) proteomics. Results Quantitative proteomic analysis identified 408 individual proteins including 26 unique proteins that changed in the treated arm during storage. Proteomic results were confirmed using immunoblot analyses and results suggested a translational control of the protein expression profile. PUNCH proteomic analysis of day 7 samples from illuminated units identified 52 unique platelet proteins that incorporated puromycin, including proteins involved in the cytoskeleton, metabolism, and signaling. Conclusion and clinical relevance This study demonstrates for the first time that platelets can synthesize proteins despite the riboflavin and UV treatment and suggests that platelets may possess a mechanism to protect their mRNA from damage by the PI treatment.