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Autoantibodies against TYMS and PDLIM1 proteins detected as circulatory signatures in Indian breast cancer patients
Author(s) -
Gupta Prachi,
Suman Shankar,
Mishra Manisha,
Mishra Sanjay,
Srivastava Nidhi,
Kumar Vijay,
Singh Pradhyumna Kumar,
Shukla Yogeshwer
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201500138
Subject(s) - autoantibody , breast cancer , titer , biomarker , medicine , cancer , serology , immunohistochemistry , antigen , cohort , oncology , immunology , antibody , pathology , biology , biochemistry
Purpose Breast cancer (BC) is the most common invasive cancer in women worldwide. Autoantibodies (AAbs) to tumor‐associated antigens (TAAs) have a great potential for the development of diagnostic biomarkers in cancer. This study was performed to identify AAbs and cognate TAAs that may improve detection of this deadly disease. Experimental design Serological proteome analysis of plasma samples of BC patients ( N = 30) and healthy controls ( N = 30) was performed to identify TAAs. Expressions of selected TAAs were also determined in breast tumor tissues ( N = 10) by immunohistochemistry. An independent validation cohort ( N = 124) was tested to determine diagnostic accuracy of selected AAbs titer by ELISA. Results Thymidylate synthase (TYMS) and C‐terminal LIM domain protein 1 (PDLIM1) were found to react more specifically with plasma samples of BC patients. Both TAAs were also found to be significantly over expressed ( p < 0.001) in breast tumor tissues compared to adjacent normal tissues. TYMS AAbs response was positively correlated ( r = 0.778, p < 0.008) with TYMS overexpression in BC tissues. TYMS and PDLIM1 AAbs titers discriminated BC from controls with a sensitivity/specificity of 57.81%/95% and 73.44%/58.33%, respectively. Conclusion and clinical relevance High titers of both TYMS and PDLIM1 AAbs were significantly more prevalent in BC cases than controls. Our data recommends further investigations for evaluating their potential for BC detection.