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Quantitative MS analysis of therapeutic mAbs and their glycosylation for pharmacokinetics study
Author(s) -
Cong Yuting,
Zhang Zhang,
Zhang Shen,
Hu Lianghai,
Gu Jingkai
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201500098
Subject(s) - pharmacokinetics , glycosylation , pharmacology , chemistry , medicine , biochemistry
Therapeutic mAbs play an important role in the treatment of a wide range of diseases. Due to their complexity, comprehensive evaluation of their pharmacokinetics has yet to be fully achieved. It is crucial to develop sensitive, accurate, reliable, and reproducible methods for quantitation of mAbs in complex samples. In addition, it is also important to evaluate the PTMs which can affect their safety and/or effectiveness. MS‐based methods provide an emerging approach for quantitation of proteins and their modification forms. In this review, we give a brief overview of quantification analysis of mAbs in complex biological samples and the characterization of N‐glycosylation by biological MS.