Premium
Quantification of decellularized human myocardial matrix: A comparison of six patients
Author(s) -
Johnson Todd D.,
Hill Ryan C.,
Dzieciatkowska Monika,
Nigam Vishal,
Behfar Atta,
Christman Karen L.,
Hansen Kirk C.
Publication year - 2016
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201500048
Subject(s) - decellularization , extracellular matrix , cadaveric spasm , proteomics , glycosaminoglycan , quantitative proteomics , matrix (chemical analysis) , chemistry , biomaterial , human blood , proteoglycan , biomedical engineering , medicine , biochemistry , chromatography , gene , physiology
Purpose The purpose of this study was to characterize and quantitatively analyze human cardiac extracellular matrix (ECM) isolated from six different cadaveric donor hearts. Experimental design ECM was isolated by decellularization of six human cadaveric donor hearts and characterized by quantifying sulfated glycosaminoglycan content (sGAG) and via PAGE. The protein content was then quantified using ECM‐targeted Quantitative conCATamers (QconCAT) by LC‐SRM analysis using 83 stable isotope labeled (SIL) peptides representing 48 different proteins. Nontargeted global analysis was also implemented using LC‐MS/MS. Results The sGAG content, PAGE, and QconCAT proteomics analysis showed significant variation between each of the six patient samples. The quantitative proteomics indicated that the majority of the protein content was composed of various fibrillar collagen components. Also, quantification of difficult to remove cellular proteins represented less than 1% of total protein content, which is very low for a decellularized biomaterial. Global proteomics identified over 200 distinct proteins present in the human cardiac ECM. Conclusion and clinical relevance In conclusion, quantification and characterization of human myocardial ECM showed significant patient‐to‐patient variability between the six investigated patients. This is an important outcome for the development of allogeneic derived biomaterials and for increasing our understanding of human myocardial ECM composition.