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Identification of six cell surface proteins for specific liver targeting
Author(s) -
Ducret Axel,
Kux van Geijtenbeek Sabine,
Röder Daniel,
Simon Sandrine,
Chin Daniel,
Berrera Marco,
Gruenbaum Lore,
Ji Changhua,
Cutler Paul
Publication year - 2015
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201400194
Subject(s) - proteome , cell , proteomics , biology , cell type , phenotype , hepatocyte , computational biology , microbiology and biotechnology , liver cell , identification (biology) , bioinformatics , gene , in vitro , biochemistry , medicine , botany
Purpose Cell surface proteins are the primary means for a cell to sense and interact with its environment and their dysregulation has been linked to numerous diseases. In particular, the identification of proteins specific to a single tissue type or to a given disease phenotype may enable the characterization of novel therapeutic targets. We tested here the feasibility of a cell surface proteomics approach to identify pertinent markers directly in a clinically relevant tissue. Experimental design We analyzed the cell surface proteome of freshly isolated primary heptatocytes using a glycocapture‐specific approach combined with a robust bioinformatics filtering. Results Using primary lung epithelial cell cultures as negative controls, we identified 32 hepatocyte‐specific cell surface proteins candidates. We used mRNA expression to select six markers that may provide adequate specificity for targeting therapeutics to the liver. Conclusions and clinical relevance We demonstrate the feasibility and the importance of conducting such studies directly in a clinically relevant tissue. In particular, the cell surface proteome of freshly isolated hepatocytes differed substantially from cultured cell lines.