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Effect of transient blood glucose increases after oral glucose intake on the human urinary proteome
Author(s) -
Li Menglin,
Zhao Mindi,
Gao Youhe
Publication year - 2015
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201400174
Subject(s) - urinary system , proteome , transient (computer programming) , chemistry , endocrinology , medicine , biochemistry , computer science , operating system
Purpose Unlike blood, which has mechanisms to maintain homeostasis, urine is more likely to reflect changes in the body. However, the urinary proteome is affected by many factors such as diet, medication, daily activities. Transient increase of blood glucose frequently occurs after the consumption of food and may be an interfering factor in biomarker studies. Experimental design In this study, a transient increase of blood glucose level was achieved by performing an oral glucose tolerance test on nine healthy human volunteers. Urine samples before and after the test were collected and analyzed by LC‐MS/MS. Results According to quantification using Progenesis LC‐MS software, only the protein neutrophil defensin 1, which was reported to participate in reducing blood glucose levels, was increased in the urine after glucose intake; and almost all urinary proteins may not be affected. Conclusions and clinical relevance Transient increase of blood glucose levels may not be a major interfering factor in biomarker studies, at least with current levels of identification and quantification.