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Targeted methods for quantitative analysis of protein glycosylation
Author(s) -
Goldman Radoslav,
Sanda Miloslav
Publication year - 2015
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201400152
Subject(s) - glycosylation , glycopeptide , quantitative proteomics , computational biology , quantitative analysis (chemistry) , glycoprotein , computer science , chemistry , proteomics , chromatography , biology , biochemistry , gene , antibiotics
Quantification of proteins by LC‐MS/MS‐MRM has become a standard method with broad projected clinical applicability. MRM quantification of protein modifications is, however, far less utilized, especially in the case of glycoproteins. This review summarizes current methods for quantitative analysis of protein glycosylation with a focus on MRM methods. We describe advantages of this quantitative approach, analytical parameters that need to be optimized to achieve reliable measurements, and point out the limitations. Differences between major classes of N‐ and O‐glycopeptides are described and class‐specific glycopeptide assays are demonstrated.