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MALDI‐TOF MS: an upcoming tool for rapid detection of antibiotic resistance in microorganisms
Author(s) -
Kostrzewa Markus,
Sparbier Katrin,
Maier Thomas,
Schubert Sören
Publication year - 2013
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201300042
Subject(s) - microorganism , clinical microbiology , antibiotic resistance , profiling (computer programming) , computer science , computational biology , biochemical engineering , identification (biology) , antibiotics , microbiology and biotechnology , biology , engineering , bacteria , genetics , operating system , botany
MALDI‐TOF MS profiling for microorganism detection has already been demonstrated in the 1990s, but has evolved to the first‐line identification method in many laboratories just during the past five years. While this application of MALDI‐TOF MS has proven its broad applicability, accuracy, robustness, and cost‐effectiveness it is of particular interest to expand the capabilities of the mass spectrometric platform. Resistance detection is the most desirable further application of MALDI‐TOF MS in microbiology, but maybe also the most challenging. Different approaches have been published regarding diverse antibiotic drugs and distinct microorganism classes. The current review shall give an overview about the developments of the recent years and their potential to get transformed in clinical useful assays in the future.