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Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID
Author(s) -
Zhu Yong,
Wu Yibo,
Jin Ke,
Lu Hui,
Liu Feng,
Guo Yueshuai,
Yan Feng,
Shi Wenbo,
Liu Yong,
Cao Xiaorong,
Hu Hongliang,
Zhu Hui,
Guo Xuejiang,
Sha Jiahao,
Li Zheng,
Zhou Zuoming
Publication year - 2013
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201200078
Subject(s) - semen , pregnancy , andrology , sperm , proteomics , proteome , infertility , semen analysis , tandem mass spectrometry , male infertility , blot , biology , medicine , bioinformatics , mass spectrometry , chemistry , genetics , gene , chromatography
Purpose To identify biochemical markers in men with idiopathic infertility and normal sperm counts. Experimental design We obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technique (ART) using 6‐plex tandem mass tag (TMT) isobaric MS. Our study design consisted of two groups: (i) The semen of six men whose sperm resulted in a clinical pregnancy following ART and (ii) six men whose semen did not result in a clinical pregnancy following ART. The results of differentiated MS were validated by Western blotting. Results and discussion A total of 2045 proteins were detected in our cohort. Twenty‐one proteins were found to be differentially expressed (>1.2‐fold) between Group 1 and Group 2. Using the results of bioinformatics analysis and Western blotting, three proteins (A2LD1, ATP1B3, and FBXO2) were shown to have the same differential pattern ( p < 0.05) that was observed in the MS analysis. Conclusions and clinical relevance Proteomics may help identity a select cohort of men with normal semen parameters and aid infertility diagnoses.