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Serum proteome profiling of pancreatitis using recombinant antibody microarrays reveals disease‐associated biomarker signatures
Author(s) -
Sandström Anna,
Andersson Roland,
Segersvärd Ralf,
Löhr Mattias,
Borrebaeck Carl A. K.,
Wingren Christer
Publication year - 2012
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201200051
Subject(s) - pancreatitis , autoimmune pancreatitis , biomarker , medicine , biomarker discovery , microarray , pancreatitis, chronic , disease , proteomics , proteome , antibody , multiplex , serology , gene expression profiling , antibody microarray , immunology , bioinformatics , biology , gene expression , gene , biochemistry
Purpose Pancreatitis is an inflammatory state of the pancreas, for which high‐performing serological biomarkers are lacking. The aim of the present study was to evaluate the use of affinity proteomics for identifying potential markers of disease and stratifying pancreatitis subtypes. Experimental design High‐content, recombinant antibody microarrays were applied for serum protein expression profiling of 113 serum samples from patients with chronic, acute, and autoimmune pancreatitis, as well as healthy controls. The sample groups were compared using supervised classification based on support vector machine analysis. Results This discovery study showed that pancreatitis subtypes could be discriminated with high accuracy. Using unfiltered data, the individual subtypes, as well as the combined pancreatitis cohort, were distinguished from healthy controls with high AUC values (0.96–1.00). Moreover, characteristic protein patterns and AUC values in the range of 0.69–0.95 were observed for the individual pancreatitis entities when compared to each other, and to all other samples combined. Conclusions and clinical relevance This study demonstrated the potential of the antibody microarray approach for stratification of pancreatitis. Distinct candidate multiplex serum biomarker signatures for chronic, acute, and autoimmune pancreatitis were defined, which could enhance our fundamental knowledge of the underlying molecular mechanisms, and potentially lead to improved diagnosis.