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Comprehensive identification of erythrocyte membrane protein deficiency by 2 D gel electrophoresis based proteomic analysis in hereditary elliptocytosis and spherocytosis
Author(s) -
Demiralp Duygu Ozel,
Peker Selen,
Turgut Burhan,
Akar Nejat
Publication year - 2012
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.201200010
Subject(s) - spectrin , hereditary spherocytosis , ankyrin , spherocytosis , proteome , membrane protein , gel electrophoresis , proteomics , peptide mass fingerprinting , chemistry , difference gel electrophoresis , two dimensional gel electrophoresis , band 3 , polyacrylamide gel electrophoresis , microbiology and biotechnology , blood proteins , biology , biochemistry , cytoskeleton , membrane , genetics , cell , immunology , gene , splenectomy , spleen , enzyme
Purpose Sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (1 DE ) may reveal qualitative or quantitative defects in red blood cell ( RBC ) membrane proteins, two‐dimensional gel electrophoresis (2 DE ) can be used for determination of the protein changes caused by the disease process in a relatively high‐throughput manner, because it permits an analysis of thousands of modified or unmodified proteins simultaneously. The principal aim of this study was to compare hereditary elliptocytosis ( HE ), hereditary spherocytosis ( HS ), and control RBC membrane protein profiles and identify proteins as a clinical marker by the sensitive methods. Experimental design RBC membrane proteins of HE , HS , and control groups were compared by 2 DE and matrix‐assisted laser desorption/ionization‐time of flight ( MALDI ‐ TOF ) MS analysis was obtained using peptide mass fingerprint for protein identification. Results Twenty proteins were identified with peptide mass fingerprint analysis and different expression levels were found in HE , HS , and controls for some proteins that includes three biomarker proteins (ankyrin, spectrin, band 3) that may have prognostic or predictive importance. Conclusions and clinical relevance 2DE of RBC proteins is a potentially valuable method for studying heritable disorders such as HE and HS . By identifying a deficiency in membrane proteins associated with the RBC cytoskeleton, the diagnosis of HE and HS can be confirmed.