Premium
Comprehensive human urine standards for comparability and standardization in clinical proteome analysis
Author(s) -
Mischak Harald,
Kolch Walter,
Aivaliotis Michalis,
Bouyssié David,
Court Magali,
Dihazi Hassan,
Dihazi Gry H.,
Franke Julia,
Garin Jérôme,
de Peredo Anne Gonzalez,
Iphöfer Alexander,
Jänsch Lothar,
Lacroix Chrystelle,
Makridakis Manousos,
Masselon Christophe,
Metzger Jochen,
Monsarrat Bernard,
Mrug Michal,
Norling Martin,
Novak Jan,
Pich Andreas,
Pitt Andrew,
BongcamRudloff Erik,
Siwy Justyna,
Suzuki Hitoshi,
Thongboonkerd Visith,
Wang LiShun,
Zoidakis Jérôme,
Zürbig Petra,
Schanstra Joost P.,
Vlahou Antonia
Publication year - 2010
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200900189
Subject(s) - proteome , context (archaeology) , biomarker discovery , proteomics , urine , biomarker , computational biology , chromatography , bioinformatics , computer science , chemistry , biology , biochemistry , paleontology , gene
Purpose : Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well‐characterized “real life” sample that can be used as reference standard in urine clinical proteomics studies. Experimental design : We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE‐MS, LC‐MS, LC‐MS/MS, 1‐D gel analysis in combination with nano‐LC MS/MS (using LTQ‐FT ultra), and 2‐DE‐MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e . proteins/peptides associated with molecular mass and detected PTMs and the relative abundance of these compounds. Results : The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in “inter‐laboratory” and “inter‐platform” data comparison is also demonstrated. Conclusions and clinical relevance : These well‐characterized urine samples are freely available upon request to enable data comparison especially in the context of biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive characterization of the urinary proteome.