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Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors
Author(s) -
Mallikarjuna Kandalam,
Sundaram Curam Sreenivasacharlu,
Sharma Yogendra,
Deepa Perinkulam Ravi,
Khetan Vikas,
Gopal Lingam,
Biswas Jyotirmay,
Sharma Tarun,
Krishnakumar Subramanian
Publication year - 2010
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200900069
Subject(s) - retinoblastoma , immunohistochemistry , carcinogenesis , downregulation and upregulation , transferrin , proteomics , biology , microbiology and biotechnology , peroxiredoxin , cancer research , apolipoprotein b , transthyretin , pathology , cancer , medicine , immunology , biochemistry , endocrinology , gene , genetics , enzyme , peroxidase , cholesterol
Purpose: To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non‐neoplastic retinas (age ranged from 45 to 89 years) as control tissues. Experimental design: 2‐DE and MALDI‐TOF‐TOF MS/MS were used to identify differentially expressed proteins. Results: Twenty‐seven distinct differentially expressed proteins were identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels of apolipoprotein A1 ( p <0.001), transferrin (TF; p <0.001), CRABP2 ( p <0.001), α‐crystallin A (CRYAA; p <0.001) were observed in RBs when compared with normal retinas and hence are consistent with the proteomic data. Immunohistochemistry was also performed for selected proteins on paraffin RB blocks to confirm protein expression. RB with invasion showed significantly higher expression by 2‐DE‐MS/MS analysis of CRABP2 ( p <0.001), peroxiredoxin 6 ( p =0.025), apolipoprotein A1 ( p <0.001), recoverin ( p <0.001). Conclusions and clinical relevance: Thus, this study provides a dynamic protein profile of RB tumors, which could provide clues to study the mechanisms of RB oncogenesis and possibly be developed as potential biomarkers for prognosis and therapy.