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Proteomics and the lung: Analysis of bronchoalveolar lavage fluid
Author(s) -
Govender Praveen,
Dunn Michael J.,
Donnelly Seamas C.
Publication year - 2009
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200900032
Subject(s) - bronchoalveolar lavage , medicine , lung , proteomics , pathology , biology , biochemistry , gene
Our knowledge of the complex bronchoalveolar lavage fluid (BALF) proteome has increased significantly over the last decade; but still, there remain many aspects of the BALF proteome that need characterization. Current proteomic methodologies resolve proteins within limited dynamic ranges: thereby, being limited in their ability to examine important areas of the BALF proteome, such as low molecular weight, low abundance proteins. To ensure proper coverage of these proteins in the BALF proteome, a refined 2‐DE standard operation protocol is presented, highlighting important issues in sample collection, sample preparation, and 2‐D DIGE analysis. It is hoped that this will help advance the field of BALF proteomics, BALFomics, which has lagged behind similar biofluids such as plasma and serum.