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Antibody microarray analysis of serum glycans in esophageal squamous cell carcinoma cases and controls
Author(s) -
Shao Changxia,
Chen Songming,
Chen Lixia,
Cobos Everardo,
Wang JiaSheng,
Haab Brian B.,
Gao Weimin
Publication year - 2009
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800245
Subject(s) - antibody , medicine , immunology , fucosylation , glycan , vitronectin , gastroenterology , microbiology and biotechnology , glycoprotein , biology , integrin , receptor
The objective of this study was to characterize specific serum glycan profile in esophageal squamous cell carcinoma (ESCC) cases and matched controls. A recently developed lectin‐based antibody array was applied to detect various cancer‐associated glycotopes in sera from 23 cases and 23 controls. Glycan levels were highly expressed in sera of ESCC patients as compared with controls. These included fucosylation level on interleukin (IL) 8; mannose level on haptoglobin; N‐acetylglucosamine levels on IL6, mucin (MUC)1, and von Willebrand factor (vWf); sialyl Lewis a (sLea) levels on blood group Lewis X, IL6, IL10, MUC1, and serum amyloid A (SAA); sialyl Tn antigen (sTn) levels on cathepsin D, gelsolin, IL10, and vWf; T antigen levels on IL8, IL10, blood group Lewis X, vitronectin, and vWf ( p <0.05). In addition, receiver‐operating characteristics (ROC) analysis showed significantly discriminal improvement between cases and controls as measured by area under ROC curve. The highest sum of sensitivity and specificity was 1.52 for carbohydrate antigen 19‐9 detection on both MUC1 and SAA, with area under ROC curves of 0.73 and 0.71, respectively. Taken together, this lectin‐based antibody microarray allows efficient profiling of variations in specific glycans on proteins in ESCC cases as compared with controls, some of which might be useful for clinical diagnosis, early detection, and/or treatment.