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Detection of urinary biomarkers for early diagnosis of acute renal allograft rejection by proteomic analysis
Author(s) -
Jia Xiongfei,
Gan Chengjun,
Xiao Ke,
He Weifeng,
Zhang Tao,
Huang Cibing,
Wu Xiongfei,
Luo Gaoxing,
Wang Xiaojuan,
Hu Jie,
Tan Jiangling,
Zhang Xiaorong,
Larsen Peter Mose,
Wu Jun
Publication year - 2009
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800209
Subject(s) - medicine , urinary system , biomarker , transplantation , urine , kidney transplantation , gastroenterology , urology , biology , biochemistry
Acute allograft rejection has been recognized as a major impediment to improved success in renal transplantation. Timely detection and control of rejection are very important for the improvement in long‐term renal allograft survival. Thus, biomarkers for early diagnosis of acute rejection are required urgently to clinical medication. This study seeks to search for such biomarker candidates by comparing patients' pre‐treatment urinary protein profiling with their post‐treatment urinary protein profiling. A total of 15 significantly and consistently down‐regulated protein candidates were identified. Among them, alpha‐1‐antichymotrypsin precursor (AACT), tumor rejection antigen gp96 (GP96) and Zn‐Alpha‐2‐Glycoprotein (ZAG) were selected for further analysis. The results indicated that Western Blot assay of AACT, GP96 and ZAG had advanced the diagnosis time of acute renal rejection by 3 days, compared with current standard clinical observation and laboratory examination. Furthermore, the double‐blind detection revealed that the accuracy, sensitivity and specificity of the diagnosis of acute renal rejection of AACT, GP96 and ZAG were 66.67%/100%/60%, 83.33%/100%/80% and 66.67%/100%/60%, respectively, and 100%/100%/100% in combination. In conclusion, urinary protein AACT, GP96 and ZAG could be a set of potential biomarkers for early non‐invasive diagnosis of the acute rejection after renal transplantation.