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Human duodenal proteome modulations by glutamine and antioxidants
Author(s) -
Thébault Sandrine,
Deniel Nicolas,
Galland Alexandra,
Lecleire Stéphane,
Charlionet Roland,
Coëffier Moïse,
Tron François,
Vaudry David,
Déchelotte Pierre
Publication year - 2010
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800175
Subject(s) - glutamine , proteome , proteomics , antioxidant , biochemistry , protein metabolism , enteral administration , inflammation , biology , chemistry , metabolism , medicine , amino acid , immunology , parenteral nutrition , gene
Abstract Purpose : Glutamine (Gln) has protective, anti‐inflammatory effects in animal models and humans. Antioxidant nutrients may exert synergistic effects on intestinal functions. Therefore, these combined nutrients may have a therapeutic potential during intestinal inflammation. This study was designed to investigate in humans the effects of a supplement composed of Gln and high‐dosed antioxidant micronutrients compared to isomolar Gln only, on duodenal proteome. Experimental design : Enteral perfusion of Gln (0.8 mmol . kg −1 . h −1 ) or supplement was performed in two groups of six healthy volunteers during 5 h before taking endoscopic duodenal biopsies. Protein expression was analyzed by 2‐DE and the relevant proteins identified by MS/MS. Results : About 1500 protein spots were revealed in both supplement and Gln conditions. Comparative proteomics analysis indicated that 11 proteins were differentially and significantly ( p ≤0.05) expressed in response to the supplement. These proteins were essentially implicated in metabolism pathways, e.g. fatty acid binding protein‐1 and 40S ribosomal protein SA expressions were downregulated while manganese superoxide dismutase and retinal dehydrogenase‐1 expressions were upregulated. Conclusions and clinical relevance : This study provides new information on human duodenal proteome and its nutritional modulation, and supports further clinical investigations designed to evaluate the effects of Gln plus antioxidants during intestinal inflammation and cancer.