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A search for novel markers of alcohol abuse using magnetic beads and MALDI‐TOF/TOF mass spectrometry
Author(s) -
Sogawa Kazuyuki,
Satoh Mamoru,
Kodera Yoshio,
Tomonaga Takeshi,
Iyo Masaomi,
Nomura Fumio
Publication year - 2009
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800174
Subject(s) - abstinence , alcohol , mass spectrometry , alcohol abuse , fibrinogen , alcohol consumption , chemistry , medicine , gastroenterology , chromatography , biochemistry , psychiatry
Objective markers are required to assess excessive alcohol consumption, which can lead to a various medical and social problems. In this study, we carried out serum peptidome analyses using the ClinProt ™ system, which consists of magnetic beads and MALDI‐TOF/TOF MS, to find novel biomarkers of alcohol abuse in 16 chronic alcoholic patients that were hospitalized for a rehabilitation program. A total of 22 peaks were found to be significantly altered during abstinence. Out of these 22 peaks, 3 peaks that had an m / z of 3000 or less and substantial peak intensities were subjected to MS/MS analysis followed by a MASCOT search. The 1466 Da and the 1616 Da peptides were upregulated on admission and were identified as fragments of fibrinopeptide A and phosphorylated fibrinopeptide A, respectively. On the other hand, the 2660 Da peptide, which was downregulated on admission and increased during abstinence, was identified as a fragment of the fibrinogen α C chain. These peaks were not detectable by the SELDI‐TOF MS ProteinChip ® system analysis. The alterations in these peaks induced by alcohol abuse were also seen in γ glutamyltransferase nonresponders. These protein fragments may be additional biomarkers for excessive alcohol drinking.