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Systematic comparison of the human saliva and plasma proteomes
Author(s) -
Yan Weihong,
Apweiler Rolf,
Balgley Brian M.,
Boontheung Pinmanee,
Bundy Jonathan L.,
Cargile Benjamin J.,
Cole Steve,
Fang Xueping,
GonzalezBegne Mireya,
Griffin Timothy J.,
Hagen Fred,
Hu Shen,
Wolinsky Lawrence E.,
Lee Cheng S.,
Malamud Daniel,
Melvin James E.,
Me Rajasree,
Mueller Michael,
Qiao Renli,
Rhodus Nelson L.,
Sevinsky Joel R.,
States David,
Stephenson James L.,
Than Shawn,
Yates John R.,
Yu Weixia,
Xie Hongwei,
Xie Yongming,
Omenn Gilbert S.,
Loo Joseph A.,
Wong David T.
Publication year - 2009
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800140
Subject(s) - saliva , proteome , ductal cells , computational biology , human proteome project , proteomics , human plasma , biology , blood proteins , salivary gland , biomarker , bioinformatics , immunology , genetics , biochemistry , chemistry , gene , chromatography , immunohistochemistry
The proteome of human salivary fluid has the potential to open new doors for disease biomarker discovery. A recent study to comprehensively identify and catalog the human ductal salivary proteome led to the compilation of 1166 proteins. The protein complexity of both saliva and plasma is large, suggesting that a comparison of these two proteomes will provide valuable insight into their physiological significance and an understanding of the unique and overlapping disease diagnostic potential that each fluid provides. To create a more comprehensive catalog of human salivary proteins, we have first compiled an extensive list of proteins from whole saliva (WS) identified through MS experiments. The WS list is thereafter combined with the proteins identified from the ductal parotid, and submandibular and sublingual (parotid/SMSL) salivas. In parallel, a core dataset of the human plasma proteome with 3020 protein identifications was recently released. A total of 1939 nonredundant salivary proteins were compiled from a total of 19 474 unique peptide sequences identified from whole and ductal salivas; 740 out of the total 1939 salivary proteins were identified in both whole and ductal saliva. A total of 597 of the salivary proteins have been observed in plasma. Gene ontology (GO) analysis showed similarities in the distributions of the saliva and plasma proteomes with regard to cellular localization, biological processes, and molecular function, but revealed differences which may be related to the different physiological functions of saliva and plasma. The comprehensive catalog of the salivary proteome and its comparison to the plasma proteome provides insights useful for future study, such as exploration of potential biomarkers for disease diagnostics.