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Protein‐induced changes during the maturation process of human dendritic cells: A 2‐D DIGE approach
Author(s) -
Ferreira Gabriela Bomfim,
Overbergh Lut,
van Etten Evelyne,
Lage Kasper,
D'Hertog Wannes,
Hansen Daniel Aaen,
Maris Michael,
Moreau Yves,
Workman Christopher T.,
Waelkens Etienne,
Mathieu Chantal
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800110
Subject(s) - microbiology and biotechnology , antigen , biology , cd14 , antigen processing , interactome , antigen presentation , proteomics , immune system , immunology , t cell , gene , genetics
Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen‐capturing cell towards a professional antigen presenting cells. In this study, a 2‐D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression upon maturation. The protein expression profile of immature and mature DCs, derived from CD14 + peripheral blood monocytes was investigated using two pH ranges (pH 4–7 and 6–9) ( n = 4). Ninety one differentially expressed spots ( p <0.01) were detected, from which we identified 74 spots (81.32%) corresponding to 41 different proteins. The proteins identified play a role in diverse processes, such as antigen processing/presentation, vesicle transport and cytoskeleton remodeling. In addition, a protein interaction network contained 29 (out of 41) proteins, suggesting that, although they functionally originate from distinct classes, these proteins are acting as a protein‐interactome. In conclusion, the proteins shown here to be altered in expression upon maturation are in line with the morphological and functional changes observed during the maturation process, providing a better understanding of the processes involved. This will open new avenues for investigating treatment regimens for immune‐associated disorders.