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The glomerular proteome in a model of chronic kidney disease
Author(s) -
Potthoff Sebastian A.,
Sitek Barbara,
Stegbauer Johannes,
Schulenborg Thomas,
Marcus Katrin,
Quack Ivo,
Rump Lars C.,
Meyer Helmut E.,
Stühler Kai,
Vonend Oliver
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200800010
Subject(s) - kidney , kidney disease , glomerulosclerosis , pathogenesis , podocyte , renal function , proteome , biology , endocrinology , medicine , albuminuria , pathology , proteinuria , bioinformatics
Adequate kidney function is crucial in sustaining vertebrate homeostasis. Certain diseases can diminish renal function and lead to end‐stage renal disease. Diabetes mellitus and hypertension are the main causes of glomerulosclerosis and albuminuria in adults. The molecular mechanisms that trigger these maladaptive changes are still unsatisfyingly described. We previously introduced 2‐D DIGE in combination with focused tissue isolation methods to analyze protein expression in glomeruli. Glomeruli, the crucial compartments in albuminuric renal diseases, were extracted using magnetic particles from subtotally nephrectomized FVB mice ( n = 6); this 5/6 nephrectomy in FVB mice is a model of chronic kidney disease. Analysis of protein expression levels from glomerular protein lysates was performed using 2‐D DIGE and compared with glomerular protein lysates from mice that underwent sham surgery. The comparison of about 2100 detectable spots between both groups revealed 48 protein spots that showed significant differential expression. Of those, 33 proteins could be identified using nanoLC‐ESI MS. The metalloproteinase meprin 1 alpha, the beta galactoside‐binding‐lectin galectin‐1 and dimethylarginine dimethylaminohydrolase 1, a key enzyme in NO metabolism, were found to be differentially regulated, thus implying a role in the pathogenesis and pathophysiology of progressive kidney disease. In conclusion, 2‐D DIGE protein analysis of smallest sample sizes from specific organ compartments provides focused protein expression results, which help in gaining an understanding of the molecular mechanisms of chronic kidney disease.