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Annexin A4: A novel molecular marker for gastric cancer with Helicobacter pylori infection using proteomics approach
Author(s) -
Lin LiLing,
Chen ChiungNien,
Lin WeiChou,
Lee PoHuang,
Chang KingJen,
Lai YoPing,
Wang JinTown,
Juan HsuehFen
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200780088
Subject(s) - helicobacter pylori , cancer , annexin , cancer research , carcinogenesis , western blot , cancer cell , angiogenesis , biology , annexin a1 , flow cytometry , microbiology and biotechnology , gene , biochemistry , genetics
Helicobacter pylori was reported to be an important risk factor for the carcinogenesis of gastric cancer. Here, we used a proteomic approach to find differentially expressed proteins between the normal and tumor tissue of gastric cancer patients infected with H. pylori . In our results, we found annexin A4 was over‐expressed in patients infected with H. pylori and was found in tumor cells, and over‐expressed in gastric cancer SCM‐1 cells after H. pylori infection. Ca 2+ can be induced by H. pylori and interact with annexin A4 Ca 2+ binding site to block the calmodulin‐activated chloride conductance activation; therefore, it produces a new environment that benefits the malignant existence of H. pylori and raises the risk for gastric cancer. We also found interleuken‐8 (IL‐8) expression levels were increased in H. pylori infected SCM‐1 cells. Combined with previous reports and our results, we summarize that the over‐expression of annexin A4 in SCM‐1 cells with H. pylori infection may subsequently induce IL‐8 which can further cause tumor angiogenesis. In this paper, we show that annexin A4 is a potential novel molecular marker for gastric cancer with H. pylori infection, and our results may provide a new insight in the development of new anti‐cancer drugs.