Premium
Human pathogenic streptococcal proteomics and vaccine development
Author(s) -
Cole Jason N.,
Henningham Anna,
Gillen Christine M.,
Ramachandran Vidiya,
Walker Mark J.
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200780048
Subject(s) - proteome , biology , proteomics , microbiology and biotechnology , antigen , identification (biology) , secretion , streptococcus , computational biology , pathogenic bacteria , secretory protein , bacteria , immunology , bioinformatics , gene , genetics , biochemistry , botany
Gram‐positive streptococci are non‐motile, chain‐forming bacteria commonly found in the normal oral and bowel flora of warm‐blooded animals. Over the past decade, a proteomic approach combining 2‐DE and MS has been used to systematically map the cellular, surface‐associated and secreted proteins of human pathogenic streptococcal species. The public availability of complete streptococcal genomic sequences and the amalgamation of proteomic, genomic and bioinformatic technologies have recently facilitated the identification of novel streptococcal vaccine candidate antigens and therapeutic agents. The objective of this review is to examine the constituents of the streptococcal cell wall and secreted proteome, the mechanisms of transport of surface and secreted proteins, and describe the current methodologies employed for the identification of novel surface‐displayed proteins and potential vaccine antigens.