Premium
Disease proteomics of endocrine disorders revealed by two‐dimensional gel electrophoresis and mass spectrometry
Author(s) -
OhIshi Masamichi,
Kodera Yoshio,
Furudate Senichi,
Maeda Tadakazu
Publication year - 2008
Publication title -
proteomics – clinical applications
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.948
H-Index - 54
eISSN - 1862-8354
pISSN - 1862-8346
DOI - 10.1002/prca.200780026
Subject(s) - proteomics , proteome , endocrine system , endocrinology , disease , biology , medicine , hormone , bioinformatics , gene , biochemistry
Endocrine disorders such as dwarfism and diabetes show abnormalities in many different organs even if a certain hormone is the primary cause of the disease. One of the aims of proteomics is to elucidate an abnormal hormone network underlying dysfunction in the disease through quantitative and qualitative proteome analyses of various organs. In a comprehensive study of the rdw rat with hereditary dwarfism, we found the accumulation of ER proteins in the rdw thyroid. Contrary to the initial notion that the dwarfism of the rat was caused by genetic mutations related to pituitary hormones, the primary cause is a missense mutation in the thyroglobulin gene. To understand at the protein level cellular damage caused by oxidative stress, we developed a proteomic method and applied to detecting protein carbonyls in various organs of a diabetes model OLETF rat. The method would provide a means toward clarifying a comprehensive view of oxidative modifications of proteins in diabetes. We review 2‐DE‐based disease proteomics of endocrine disorders in general, with particular attention paid to our proteome projects by a 2‐DE method with an agarose IEF gel in the first dimension (agarose 2‐DE) and LC‐MS/MS.